HPLC Analysis of Tetracycline Antibiotics on Ascentis® Phenyl

HPLC Analysis of Tetracycline Antibiotics on Ascentis® Phenyl

Conditions

column Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 µm particles (581616-U)
column temp. 35 °C
mobile phase [A] 0.1% ammonium acetate; [B] acetonitrile with 0.1% ammonium acetate; (80:20, A:B)
flow rate 1.0 mL/min
sample 50 µg/mL each in 80:20, water:methanol
injection 10 µL
detector UV, 353 nm

Description

Categories Analytical Chromatography, Pharmaceutical Analysis, Antibiotics, Antibiotics, Antimicrobials
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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