HPLC Analysis of Tetramisole Enantiomers and Related Compounds on Astec® CYCLOBOND® I 2000 DMP

HPLC Analysis of Tetramisole Enantiomers and Related Compounds on Astec® CYCLOBOND® I 2000 DMP

Conditions

column Astec CYCLOBOND I 2000 DMP 10 cm X 2.1 mm
column temp. 35 °C
mobile phase (A) 100 mM ammonium acetate, pH 5 with acetic acid (B) acetonitrile, 90:10, v/v
flow rate 0.2 mL/min
pressure 360 psi
sample 100 ug/mL tetramisole and 50 ug/mL each cocaine and aminorex in water:methanol, 80:20, v/v
injection 2 µL
detector UV, 220 nm

Description

Analysis Note Cocaine is often found laced with levamisole (one of the tetramisole enantiomers) and aminorex is a metabolite of levamisole known to cause serious vascular damage in drug abusers. These compounds are readily separated using the Astec CYCLOBOND column - a derivatized cyclodextrin column only offered by Supelco. What is really interesting about this separation is that it shows the ability of chiral HPLC columns to separate enantiomers (here, dexamisole and levamisole) by chiral discrimination, as well as non-chiral discrimination in the same analysis. The compounds aminorex and cocaine are not enantiomers of each other or of tetramisole. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Antihelminthic, Drugs of Abuse
Featured Industry Pharmaceutical (small molecule)
Legal Information Astec is a registered trademark of Sigma-Aldrich Co. LLC, CYCLOBOND is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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