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HPLC Analysis of Thyroglobulin, gamma-Globulins, Ovalbumin, Myoglobin, and Poly-DL-Alanine on Zenix® SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles

HPLC Analysis of Thyroglobulin, gamma-Globulins, Ovalbumin, Myoglobin, and Poly-DL-Alanine on Zenix® SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles

Conditions

column Zenix(R) SEC-150, 30 cm x 7.8 mm I.D., 3 µm particles (Z777018)
column temp. 25 C
mobile phase 150 mM potassium phosphate monobasic, pH 7.0 (adjusted with potassium hydroxide)
flow rate 0.7 ml/min
pressure 890 psi (61.4 bar)
sample 1 g/L each (except uracil, 0.1 g/L ) in mobile phase A
injection 1 µl
detector UV, 214 nm

Description

Analysis Note This application demonstrates the suitability of Zenix SEC-150 for the separation of proteins. Separation is by inclusion into the pore volume with larger molecules being excluded and therefore eluting earlier.
Categories Analytical Chromatography, Clinical, Clinical Antibodies, Antibiotics, Antimicrobials, Proteins, proteins
Featured Industry Clinical
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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