HPLC Analysis of Thyroxine Enantiomers on Astec® CHIROBIOTIC® TAG

HPLC Analysis of Thyroxine Enantiomers on Astec® CHIROBIOTIC® TAG

Conditions

column CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 µm particles (14024AST)
column temp. 25 °C
mobile phase 1% ammonium formate in methanol
flow rate 1 mL/min
pressure 826 psi (57 bar)
sample each enantiomer 0.1 mg/L in methanol
injection 10 µL
detector UV, 220 nm

Description

Analysis Note Baseline resolution of the chiral enantiomers of DL-Thyroxine is achieved by polar ionic mode on the Astec CHIROBIOTIC TAG with the use of an LC/MS-compatible mobile phase.
Categories Analytical Chromatography, Chiral Applications, Clinical, Hormones, Clinical, Forensic & Toxicology, hormones
Featured Industry Clinical
Life Science and Biopharma
Legal Information CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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