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HPLC Analysis of Triacylglycerols in Olive Oil on Discovery® HS C18 after Sample Prep using Discovery® DSC-Si (Acetone-Acetonitrile Mobile Phase, ELSD Detection)

HPLC Analysis of Triacylglycerols in Olive Oil on Discovery® HS C18 after Sample Prep using Discovery® DSC-Si (Acetone-Acetonitrile Mobile Phase, ELSD Detection)

Conditions

SPE tube/cartridge Discovery DSC-Si, 1 gm/6 mL (52656-U)
condition 6 mLhexane
sample addition 0.12 g oil in 0.5 mL hexane
elution 10 mL hexane-diethyl ether 87-13 (v/v)
eluate post-treatment evaporation to dryness, reconstituted into 2 mL acetone
column Discovery HS C18, 25 cm x 4.6 mm I.D., 5 µm particles (568523-U)
column temp. 30 C
mobile phase [A] acetone; [B] acetonitrile; (60:40, A:B)
flow rate 1.5 ml/min
pressure 914 psi (63 bar)
injection 5 µl
detector ELSD

Description

Analysis Note Olive oil was pre-cleaned using Silica SPE, analyzed as 5% sample in acetone (w/v).
Categories Analytical Chromatography, Glycerols, Fatty Acids, FAMEs
Featured Industry Food and Beverages
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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