HPLC Analysis of Tricyclic Antidepressants on Ascentis® C8

HPLC Analysis of Tricyclic Antidepressants on Ascentis® C8

Conditions

column Ascentis C8, 15 cm x 2.1 mm I.D., 3 µm particles (581402-U)
column temp. 35 C
mobile phase [A] 25 mM ammonium phosphate dibasic (pH 7.0 with phosphoric acid): [B] methanol; (25:75, A:B)
flow rate 0.2 ml/min
sample as indicated in 60:40 water:methanol
injection 10 µl
detector UV, 254 nm

Description

Categories Analytical Chromatography, Clinical, Pharmaceutical Analysis, antidepressants, Antidepressants
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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