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HPLC Analysis of Tricyclic Antidepressants on Ascentis® Express 2.7 μm RP-Amide

HPLC Analysis of Tricyclic Antidepressants on Ascentis® Express 2.7 μm RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 µm particles (53913-U)
column temp. 35 C
mobile phase [A] 25 mM ammonium phophate pH 7.0 with 30% Phosporic Acid; [B] methanol; (30:70, A:B)
flow rate 0.3 ml/min
pressure 3162 psi (218 bar)
sample Amitriptyline, Clomipramine, Desipramine, Doxepin,    Imipramine, Norclomipramine, Nortriptyline, 50          µg/mL in 60:40 mobile phase A:mobile phase B
injection 0.5 µl
detector UV, 254 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express 2.7 μm RP-Amide for the separation of tricylcic antidepressants. The phase chemistry is well suited to this class of hydrophobic bases and offers improved resolution and peak shape compared to traditional reversed phase C18 analysis. The core shell particle shows an increased speed of analysis compared to the standard Ascentis application.
Categories Analytical Chromatography, Antidepressants
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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