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HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery® C18 after SPE using Discovery® DSC-18

HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery® C18 after SPE using Discovery® DSC-18

Conditions

sample preparation SPE (Solid Phase Extraction)
SPE tube/cartridge Discovery DSC-18, 100 mg/1 mL (52602-U)
column Discovery C18, 15 cm x 4.6 mm, 5 µm preceded by a 2 cm C18 guard column and 0.5 µm frit filter (504955)
column temp. ambient
mobile phase [A] acetonitrile: [B] methanol: [C] 25 mM potassium phosphate, monobasic (pH 7 with triethlylamine) (45:25:30, A:B:C)
flow rate 1 ml/min
injection 50 µL, diluted porcine serum extract
detector UV, 254 nm

Description

Categories Analytical Chromatography, SPE Applications, Drugs of abuse, Pharmaceutical & Clinical applications
Featured Industry Clinical
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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