HPLC Analysis of Tryptic Digest of Protein Mixture on BIOshell™ A160 2.7 μm Peptide C18

HPLC Analysis of Tryptic Digest of Protein Mixture on BIOshell™ A160 2.7 μm Peptide C18

Conditions

column BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 µm particles (66905-U)
column temp. 35 °C
mobile phase [A] 0.1% formic acid in water; [B] 0.4% formic acid in 25:75 (v/v) water:acetonitrile
gradient 6 to 70% B in 40 min
flow rate 0.3 mL/min
sample 10 pmol/L in 0.1% formic acid
injection 2 µL
detector ESI(+), TOF

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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