HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis® Express C18 after Phospholipid Removal using HybridSPE®-Phospholipid

HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis® Express C18 after Phospholipid Removal using HybridSPE®-Phospholipid

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix Rat plasma spiked with verapamil and metabolites at 10 ng/mL
SPE well plate HybridSPE-Precipitation 96-well Plate, 50 mg/well (575656-U)
sample addition 100 µl spiked rat plasma followed by 300 µl 1% formic acid in acetonitrile
elution apply vacuum
column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 µm particles (53822-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile, pH 2.55 adjust with formic acid (30:70, A:B)
flow rate 0.6 ml/min
injection 1 µl
detector TOF/MS

Description

Categories Analytical Chromatography, SPE Applications, Clinical, Pharmaceutical Analysis, Anithypertensive, antihypertensives, antihistamines, Antihypertensives, Pharmaceutical & Clinical applications
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, HybridSPE is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS, application for SPE

Materials

     
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