HPLC Analysis of Vitamins B1, B6 and B12 using Ascentis® Express RP-Amide

HPLC Analysis of Vitamins B1, B6 and B12 using Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 µm particles (50773-U)
column temp. 30 °C
mobile phase [A] 0.02 M Ammonium Formate, pH = 3.0; [B] acetonitrile
gradient 0-50% B in 1 min. Hold at 50% B until 1.5 min
flow rate 1.5 mL/min
pressure 1639 psi (113 bar)
sample Dissolved in 50/50 water/ACN
injection 1 µL
detector UV, 276 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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