HPLC Analysis of Vitamins, Water Soluble, on Discovery® C8

HPLC Analysis of Vitamins, Water Soluble, on Discovery® C8

Conditions

column Discovery C8, 15 cm x 4.6 mm I.D., 5 µm particles (59353-U)
column temp. 35 °C
mobile phase [A] 50 mM potassium phosphate, monobasic, pH 4.5 to methanol: [B] 50 mM potassium phosphate, monobasic, pH 4.(30:70) in 12 min
flow rate 1 mL/min
injection 1 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Food and Beverages
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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