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HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express F5

HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express F5


column Ascentis Express F5, 5 cm x 3.0 mm I.D., 2.7 µm particles (53576-U)
column temp. 35 C
mobile phase [A] 20 mM potassium phosphate, dibasic, pH 6.2; [B] acetonitrile
gradient 0% B for 1.5 min, 0-10% over 0.5 min; to 15% B in 1.5 min, 2 min re-equilibration at 0% B
flow rate 1.5 ml/min
pressure 4931 psi (340 bar)
sample all vitamins in water, 10 µg/mL for nicotinic acid and folic acid, 12 µg/mL for thiamine hydrochloride and cyanocobalamin, (20 µg/mL for pyridoxine, 30 µg/mL for riboflavin, 40 µg/mL for niacineamide, 100 µg/mL for calcium pantothenate, 120 µg/mL for biotin)
injection 5 µl
detector UV, 210 nm


Analysis Note A mix of 9 water-soluble B-vitamins was separated on Ascentis Express F5 column
Categories Analytical Chromatography, Pharmaceutical Analysis, Anticonvulsant/antiepiletic/antiseizure, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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