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HPLC Analysis of Withania Extract on Ascentis® Express F5

HPLC Analysis of Withania Extract on Ascentis® Express F5


column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53569-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile
gradient 20 to 100% B in 5 min; held at 100% B for 0.5 min
flow rate 0.3 ml/min
pressure 2750 psi (190 bar)
sample 20 mg/mL in water:acetonitrile (50:50); sonicate for 15 minutes; filter 0.45 µm; dilute to water:acetonitrile (80:20)
injection 5 µl
detector UV, 227 nm


Analysis Note This application demonstrates the suitability of Ascentis Express F5 for the efficient separation of withania extract.
Categories Analytical Chromatography, Herbal medicines
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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