Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Withania Standards on Discovery® C18

HPLC Analysis of Withania Standards on Discovery® C18

Conditions

column Discovery C18, 25 cm x 4.6 mm I.D., 5 µm particles (504971)
column temp. 27 C
mobile phase [A] 1 mM potassium phosphate, monobasic; [B] acetonitrile
gradient 5 to 45% B in 18 min; 45 to 80% B in 7 min; held at 80% B for 3 min
flow rate 1.5 ml/min
pressure 220 bar (3200 psi)
sample 20 µg/mL in water: acetonitrile (86:14)
injection 20 µl
detector UV, 227 nm

Description

Analysis Note This application demonstrates the suitability of Discovery C18 for the efficient separation of withania standards by the USP monograph.
Categories Analytical Chromatography, Herbal medicines
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links