HPLC Analysis of Z-Drugs on Ascentis® Express C18 2.0 μm

HPLC Analysis of Z-Drugs on Ascentis® Express C18 2.0 μm

Conditions

column Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.0 µm particles (50819-U)
column temp. 30 °C
mobile phase [A] 10 mM ammonium formate pH 3.0 with formic acid in 90:10, water:acetonitrile; [B] 10 mM ammonium formate pH 3.0 with formic acid in 10:90, water:acetonitrile;
gradient 0 to 100% B in 3.0 min; held at 100% B for 0.5 min
flow rate 0.6 mL/min
pressure 5405 psi (373 bar)
sample 10 µg/mL in 96:4, water:methanol
injection 0.5 µL
detector MS, ESI+, combined SIR

Description

Analysis Note An rapid method for the siµLtaneous determination of the Z-drugs or sleep aids: zopiclone, zolpidem, and zaleplon is presented here. The need for greater analytical capacity and throughput for the analysis of sleep aid medicines (Z-drugs) in forensic toxicology laboratories can be met by the use of fast Ascentis Express 2.0 micron Fused Core UHPLC Columns.
Categories Analytical Chromatography, Anxiolytics, Sedatives
Featured Industry Clinical
Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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