HPLC Chiral Analysis of Levamisole on Astec® Cyclobond® I 2000 DMP

HPLC Chiral Analysis of Levamisole on Astec® Cyclobond® I 2000 DMP

Conditions

column ASTEC CYCLOBOND I 2000 DMP 25 cm x 4.6 mm I.D., 5mum particles (20724AST)
column temp. 35 C
mobile phase (A) 100 mM ammonium acetate, pH 5 with acetic acid (B) acetonitrile, 80:20, v/v
flow rate 1.0 ml/min
pressure 1600 psi (110 bar)
sample 200 ug/mL tetramisole in mobile phase A:methanol, 80:20, v/v
injection 10 µl
detector UV, 230 nm

Description

Analysis Note Cyclobond I 2000 DMP is effective at chiral recognition of dexamisole from levamisole forµlations.  This method appears to be a suitable starting point for further development towards the analysis of levamisole chiral purity.  The method is also a suitable replacement for published methods utilizing Cyclobond I 2000 SN which is no longer available.
Categories Analytical Chromatography, Forensics and Toxicology, chemotherapy drugs, Chemotherapy drugs
Featured Industry Forensics and Toxicology
Legal Information Astec is a registered trademark of Sigma-Aldrich Co. LLC, CYCLOBOND is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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