HPLC Separation of an Oligonucleotide and Impurities on Proteomix® SAX-NP5 versus Porous SAX

HPLC Separation of an Oligonucleotide and Impurities on Proteomix® SAX-NP5 versus Porous SAX

Conditions

column Proteomix SAX-NP5, 15 cm x 4.6 mm I.D., 5 µm particles (Z777228)
mobile phase (A) 0.025 M Tris buffer pH9; (B) A + 1.0 M LiCi
gradient 30 to 75% B in 45 min
flow rate 1.0 mL/min
sample 50-mer Oligo
injection 50 µL
detector UV, 260 nm

Description

Categories Analytical Chromatography, Nucleic Acids
Featured Industry Forensics and Toxicology
Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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