HPLC Separation of D-sphingosine and related compounds on Ascentis® Express C18

HPLC Separation of D-sphingosine and related compounds on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm X 2.1 mm, 2.7um particles (53823-U)
column temp. 35 C
mobile phase (A) Water; (B) Acetonitrile, each with 0.1% formic acid/10 mM ammonium formate
gradient 70% B for 8 min, 0.5 min to 90%B, hold for 1.5 min, back to 70% in 0.5 min, hold for 3.5 min
flow rate 0.3 ml/min
pressure 3900 psi (269 bar)
sample 10 ng/mL in 50% methanol/0.1% formic acid
injection 1 µl
detector D-erythro-Sphingosine 300.3/282.3  MRM(+) ; L-threo-Sphingosine 300.3/282.3 MRM(+) ; D-erythro-Dihydrosphingosine 302.3/284.3 MRM(+)
detector ESI-MS (+)

Description

Analysis Note HPLC Separation of D-erythro- and L-threo-Sphingosine and its related compounds.
Categories Analytical Chromatography, Lipids
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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