HPLC Size-Exclusion Analysis of Chimeric Monoclonal Antibody on TSKgel® UltraSW Aggregate

HPLC Size-Exclusion Analysis of Chimeric Monoclonal Antibody on TSKgel® UltraSW Aggregate

Conditions

column TSKgel UltraSW Aggregate, 30 cm x 7.8 mm I.D., 3 µm particles (822856)
mobile phase 0.2 M phosphate buffer, pH 6.7 + 0.05% sodium azide
flow rate 0.8 mL/min
sample Monoclonal antibody 2 (mouse-human chimeric IfG, Erbitux)
injection 10 µL
detector UV, 280 nm

Description

Analysis Note Following their production, therapeutic preparations of monoclonal antibodies are analyzed by size-exclusion chromatography to ensure that the aggregate level is below a specified value. Globular proteins are excluded from the TSKgel® UltraSW Aggregate column at a molecular mass of about two million dalton, which provides the highest resolution for protein aggregates of any of the TSKgel SW mAb series columns from Tosoh Bioscience.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
suitability application for HPLC

Materials

     
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