HPLC (Size Exclusion) Analysis of Proteins on Zenix®-C SEC-300, Effect of DTT and EDTA

HPLC (Size Exclusion) Analysis of Proteins on Zenix®-C SEC-300, Effect of DTT and EDTA

Conditions

column Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 µm particles (Z777092)
column temp. 25 °C
mobile phase (A) 150 mM sodium phosphate buffer, pH 7.0 (B) 150 mM sodium phosphate buffer, pH 7.0 with 2 mM DTT, 0.1 mM EDTA (C) 150 mM sodium phosphate buffer, pH 7.0 with 2 mM DTT, 0.1 mM EDTA
flow rate 1 mL/min
sample proteins
injection 10 µL
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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