HPLC Size-Exlusion Analysis of mAb Monomer, Dimer, and Higher-Order Aggregates on TSKgel® SuperSW mAb HTP

HPLC Size-Exlusion Analysis of mAb Monomer, Dimer, and Higher-Order Aggregates on TSKgel® SuperSW mAb HTP

Conditions

column TSKgel SuperSW mAb HTP 4 µm, 15 cm x 4.6 mm I.D., 4 µm particles (822855)
column temp. 25 °C
mobile phase 0.2 M phosphate buffer, pH 6.7 + 0.05% sodium azide
flow rate 0.5 mL/min and 0.35 mL/min
sample Monoclonal antibody 2 (mouse-human chimeric IfG, Erbitux)
injection 5 µL
detector UV, 280 nm

Description

Analysis Note Size-exclusion chromatography is now routinely used in the quality control of commercial monoclonal antibody (mAb) preparations. In this example, a mAb monomer, dimer and higher order aggregates are easily separated within 5 minutes on a 15 cm x 4.6 mm ID TSKgel® SuperSW mAb HTP column at a high flow rate of 0.35 mL/min. After Increasing the flow rate to 0.5 mL/min the monomer elutes within 3 minutes from the column.
Categories Analytical Chromatography, Proteins, Peptides
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
suitability application for HPLC

Materials

     
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