HPLC Analysis of Horny Goat Weed Standards using Ascentis® Express C18

HPLC Analysis of Horny Goat Weed Standards using Ascentis® Express C18

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 5 µm particles (50537-U)
column temp. 35 °C
mobile phase [A] 0.1% acetic acid in water; [B] 0.1% acetic acid in acetonitrile;
gradient 5% B held for 0.75 min; to 40% B in 3.75 min; to 100% B in 3.5   min; 100% B held for 2 min
flow rate 1.5 mL/min
pressure 2120 psi (146 bar)
sample 50 µg/mL 70:30, water:methanol
injection 5 µL
detector UV, 250 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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