Improved HPLC Selectivity of Discovery® BIO Wide Pore RP Phases

Improved HPLC Selectivity of Discovery® BIO Wide Pore RP Phases

Conditions

column Discovery BIO Wide Pore C18, 15 cm x 4.6 mm I.D., 5 µm particles vs. competitive wide pore C18 column of same dimensions (568222-U)
column temp. 30 °C
mobile phase [A] 0.1% TFA in 80:20 water:acetonitrile; [B] 0.1% TFA in 66:34 water:acetonitrileGradient of 0 to 100% B in 14 min. after 1 min. delay
gradient 0 to 100% B in 14 min. after 1 min. delay
flow rate 1 mL/min
sample peptide mixture (Sigma P2693), ~0.25 mug each peptide in 0.1% TFA
injection 10 µL
detector UV, 220 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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