LC/MS Analysis of Acesulfame K and Aspartame in Soft Drinks on SeQuant ZIC-HILIC

LC/MS Analysis of Acesulfame K and Aspartame in Soft Drinks on SeQuant ZIC-HILIC

Conditions

column Sequant ZIC-HILIC, PEEK, 100 x 2.1 mm, 3.5 µm, 100 Ã… (1.50441.0001)
column temp. 40 °C
mobile phase [A] acetonitrile; [B] ammonium acetate;
gradient 0% B increasing at 10% B/min from 0 to 5 min; 0% B at 5.01 min; held at 0% B for 5 min
flow rate 0.4 mL/min
pressure 1267 psi (8.8 MPa)
injection 2 µL
detector Shimadzu LC-2010 Evolution, detector voltage: 2.0 kV
detector heat block temp: 200 °C; CDL temp:200 °C; (SIM mode: m/z 162.0 (Acesulfame K), and 295 (Aspartame))

Description

Featured Industry Food and Beverages
Other Notes Sample Prep: degassed soft drink diluted 1:10 with initial mobile phase
suitability application for LC-MS

Materials

     
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