LC/MS Analysis of Chloramphenicol in Shrimp on Ascentis® Express C18 after Dispersive SPE (QuEChERS) Cleanup using Supel™ QuE Z-Sep+

LC/MS Analysis of Chloramphenicol in Shrimp on Ascentis® Express C18 after Dispersive SPE (QuEChERS) Cleanup using Supel™ QuE Z-Sep+

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix 1.0 gshrimp, spiked at 0.3 ng/g
extraction 9 mL acetonitrile, then 90 µL of formic acid
extraction process post treatment: 8 mL supernatant evaporated to dryness and reconstituted with 1 mL water
clean-up process clean-up volume: 9 mL
clean-up tube Supel™ Que Z-Sep+ tube, 12 mL, 500 mg (55296-U)
column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 µm particles (53822-U)
column temp. ambient
mobile phase [A] water; [B] acetonitrile
gradient 15% B for 0.1 min, 15-80% in 1.9 min, held at 80% for 1.5 min, 80-15% in 0.5 min, at 15% for 3 min
flow rate 0.5 mL/min
injection 10 µL
detector MS, ESI(+), MRM, m/z 320.9/151.9, 325.9/157.0

Description

Analysis Note This application demonstrates the superior sample cleanup with the QuEChERS approach using the unique Supel QuE Z-Sep+ sorbent. High resolution and rapid analysis was provided by the Ascentis Express 2.7 μm C18 column.
Categories Analytical Chromatography, SPE Applications, Antibodies, Antibiotics/antimicrobials
Featured Industry Environmental
Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS, application for SPE

Materials

     
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