LC/MS Analysis of Equine Performance-Enhancing Dopants GABA and AICAR on Ascentis® Express HILIC

LC/MS Analysis of Equine Performance-Enhancing Dopants GABA and AICAR on Ascentis® Express HILIC

Conditions

column Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 µm particles (53939-U)
column temp. 35 C
mobile phase [A] 5 mM ammonium formate in acetonitrile:water (95:5); [B] 5 mM ammonium formate in acetonitrile:water (70:30)
gradient 0 to 70% B in 3 min, held for 1 min
flow rate 0.4 ml/min
sample 1 µg/mL in acetonitrile
injection 2 µl
detector MS, ESI+/ESI-

Description

Analysis Note This study focuses on the analysis of possible dopants used in equine racing, polar compounds requiring HILIC chromatographic separation. The rapid separation of four such compounds is shown here on an Ascentis Express HILIC column. The highest grade solvents provided clean, robust operation. Cerilliant ethyl-β-D-glucuronide CRM was used for reliable quantification.
Categories Analytical Chromatography, Neurotransmitters
Featured Industry Forensics and Toxicology
Veterinary
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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