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LC/MS Analysis of Erythromycin Analogs on Ascentis® Express HILIC

LC/MS Analysis of Erythromycin Analogs on Ascentis® Express HILIC


column Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 µm particles (53981-U)
column temp. 35 C
mobile phase [A] 100 mM ammonium formate, pH 3.0 (titrated with formic acid); [B] acetonitrile; (10:90, A:B)
flow rate 1.0 ml/min
pressure 1247 psi (86 bar)
injection 5 µl
detector ESI(+); SIR


Analysis Note This application shows the suitability of Ascentis Express Silica columns for the analysis of erythromycin analogs in aqueous normal phase/HILIC mode of chromatography. Other relatively hydrophobic aminoglycosides have also been shown to separate under these conditions.
Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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