LC/MS Analysis of Ethanol Metabolites Ethyl Sulfate and Ethyl-β-D-Glucuronide on Ascentis® Express RP-Amide

LC/MS Analysis of Ethanol Metabolites Ethyl Sulfate and Ethyl-β-D-Glucuronide on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 µm particles (53913-U)
column temp. 35 C
mobile phase 0.1% (v/v) formic acid in water
flow rate 0.4 ml/min
pressure 3066 psi (211 bar)
sample 2 µg/mL in 99:1, water:methanol
injection 2 µl
detector MS, ESI(-), combined XIC, 125.0 m/z, 221.1 m/z

Description

Analysis Note The compounds ethyl sulfate and ethyl-β-D-glucuronide are metabolites of ethanol. Their analysis is of clinical interest as biomarkers or indicators of alcohol-induced liver disease (ALD). The HPLC analysis of these compounds using reversed-phase mode using conventional C18 chemistries suffers from poor retention due to the compounds′ polar nature. However, polar compound retention is improved by using the Ascentis Express RP-Amide, a polar-embedded phase, while still keeping the reversed-phase mechanism. Cerilliant CRMs provided reliable quantification.
Categories Analytical Chromatography, Forensics and Toxicology, Clinical, Alcohols, Drugs of Abuse, drugs of abuse, alcohols
Featured Industry Clinical
Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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