LC/MS Analysis of Flavonoids on Chromolith® FastGradient RP-18 endcapped

LC/MS Analysis of Flavonoids on Chromolith® FastGradient RP-18 endcapped

Conditions

column Chromolith FastGradient RP-18, 5 cm x 2.0 mm I.D., 2 µm particles (1.52007.0001)
column temp. 25 °C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient 5 to 95% B in 4 min, held at 95% B for 0.5 min, to 5% B in 0.5 min, held at 5% for 2 min
flow rate 0.4 mL/min
pressure 677-245 psi (47 - 17 bar)
sample catechin 317 ppb, epicatechin 270 ppb, quercitin 198 ppb, hesperetin 177 ppb in water
injection 1 µL
detector ESI-MS(+) (m/z range 100-455)

Description

Categories Analytical Chromatography, Dyes
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for LC-MS

Materials

     
Related Links