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LC/MS Analysis of Green Tea Components on Chromolith® FastGradient RP-18 endcapped

LC/MS Analysis of Green Tea Components on Chromolith® FastGradient RP-18 endcapped

Conditions

column Chromolith FastGradient RP-18, 5 cm x 2.0 mm I.D., 2 µm particles (1.52007.0001)
column temp. 25 C
mobile phase [A] 0.1% formic acid in acetonitrile; [B] 0.1% formic acid in water
gradient 100% B for 0.6 min, to 72.5% B in 7.4 min, held at 72.5% for 1 min
flow rate 0.4 µL/min
pressure 605 psi (42 bar)
injection 1 µl
detector ESI-MS(+) (m/z range 150-950), BPC and overlay of EICs)

Description

Categories Analytical Chromatography, Natural products
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes Sample Prep: 1.75 g of green tea was extracted for three minutes using 100 mL of boiling water. Prior to injection both samples were centrifuged at 4500 rpm for 10 min and cleaned using a 0.45 um syringe filter.
suitability application for LC-MS

Materials

     
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