LC/MS Analysis of Illicit Drugs and Metabolites on Ascentis® Express RP-Amide

LC/MS Analysis of Illicit Drugs and Metabolites on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 µm particles (53913-U)
column temp. 35 C
mobile phase 10 mM ammonium formate in water:acetonitrile (75:25)
flow rate 0.2 ml/min
sample each compound 200 ng/mL in mobile phase
injection 2 µl
detector ESI+, MRM (see Figure for transitions)

Description

Analysis Note These illicit drugs are routinely tested in drug screens for forensic and pre-employment. Their rapid separation was achieved on an Ascentis Express RP-Amide column. Cerilliant CRMs provide reliable quantification.
Categories Analytical Chromatography, Forensics and Toxicology, Drugs of Abuse, drugs of abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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