LC/MS Analysis of Methadone and Metabolites EDDP and EMDP on Ascentis® Express RP Amide

LC/MS Analysis of Methadone and Metabolites EDDP and EMDP on Ascentis® Express RP Amide

Conditions

column Ascentis Express RP Amide, 10 cm x 2.1 mm I.D., 2.7 µm particles (53913-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium formate in water, pH 3.6; [B] 10 mM ammonium formate in acetonitrile, pH 3.6; (65:35, A:B)
flow rate 0.5 ml/min
sample 200 µg/L in 25:75, water: acetonitrile
injection 0.5 µl
detector ESI(+), m/z 100-1000

Description

Categories Analytical Chromatography, Forensics and Toxicology, analgesics, Drugs of Abuse, drugs of abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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