LC/MS Analysis of Omeprazole and Metabolites on Ascentis® Express RP Amide (pH 2.7)

LC/MS Analysis of Omeprazole and Metabolites on Ascentis® Express RP Amide (pH 2.7)

Conditions

column Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 µm particles (53911-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate in water, pH 2.7; [B] 10 mM ammonium formate in methanol, pH 2.7; (65:35, A:B)
flow rate 0.3 mL/min
sample 1 mg/L in mobile phase
injection 1 µL
detector ESI(+), m/z 50-400

Description

Analysis Note This application demonstrates the suitability of the Ascentis Express RP Amide for the analysis of omeprazole metabolites.
Categories Analytical Chromatography, Clinical, antiulcer compounds, Antiulcer Compounds
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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