LC/MS Analysis of Omeprazole and Metabolites in Plasma on Ascentis® Express RP Amide after Sample Prep using HybridSPE®-Phospholipid

LC/MS Analysis of Omeprazole and Metabolites in Plasma on Ascentis® Express RP Amide after Sample Prep using HybridSPE®-Phospholipid

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix rat plasma spiked with omeprazole, phenacetin and metabolites at 2 µg/mL
SPE well plate HybridSPE-Phospholipid, 96-well plate, 15 mg/0.8 mL (575656-U)
sample addition 100 µL plasma to each well, followed by 300 mL of 1% formic acid in acetonitrile
elution apply vacuum
eluate post-treatment eluate was collected, evaporated, and reconstituted in mobile phase.
column Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 µm particles (53911-U)
column temp. 35 °C
mobile phase [A] water, 10 mM ammonium formate, pH 3.4; [B] methanol, 10 mM ammonium formate, pH 3.4; (65:35, A:B)
flow rate 0.3 mL/min
injection 1 µL
detector ESI(+), m/z = 50-400

Description

Analysis Note Sample was vorlexed and centrifuged for 2 minutes at 15000 rpm. Supemate was then passed through HybridSPE 96 well plate. Elutent was collected, evaporated and reconstituted in mobile phase.
Application This application demonstrates the suitability of HybridSPE and the Ascentis Express RP Amide for the analysis of omeprazole metabolites.
Categories Analytical Chromatography, Clinical, SPE Applications, Antiulcer Compounds, Antiulcer compounds, antiulcer compounds
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, HybridSPE is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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