LC-MS Analysis of Pain Management Opiates using Ascentis® Express Biphenyl

LC-MS Analysis of Pain Management Opiates using Ascentis® Express Biphenyl

Conditions

column Ascentis Express Biphenyl, 5 cm x 2.1mm, 2.7 µm particles (64057-U)
column temp. 30 °C
mobile phase [A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid;
gradient 10 to 20% B in 1.25 min; to 95% B in 3.75 min
flow rate 0.3 mL/min
pressure 2990 psi (206 bar)
sample 50-500 ng/mL in 99:1, water:methanol
injection 2 µL
detector MS-TOF, ESI+

Description

Analysis Note This application demonstrates the separation of 13 pain managment opiates on the Ascentis Express Biphenyl using an LC-TOF mass spectrometer. This application demonstrates the scalability of this method to 50 x 2.1 mm column geometry using the 2.7 um biphenyl phase. The method provides baseline resolution of all isobaric sets of interest.
Categories Analytical Chromatography, Pharmaceuticals, Opiate
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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