LC/MS Analysis of Peptides, Hydrophobic, on Ascentis® RP-Amide

LC/MS Analysis of Peptides, Hydrophobic, on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 10 cm x 2.1 mm I.D., 5 µm particles (565304-U)
column temp. ambient
mobile phase [A] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:water; [B] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:acetonitrile
gradient 15 to 45% B in 15 min
flow rate 0.2 mL/min
sample as indicated in 10 mM formic acid
injection 4 µL
detector ESI (+)

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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