LC/MS Analysis of Phosphatidylethanol Metabolites on Titan™ PFP 1.9 μm

LC/MS Analysis of Phosphatidylethanol Metabolites on Titan™ PFP 1.9 μm

Conditions

column Titan PFP, 5 cm x 2.1 mm I.D., 1.9 µm particles (569677-U)
column temp. 50 °C
mobile phase 5 mM ammonium formate in [A] water; [B] methanol; [C] acetonitrile; (20:40:40, A:B:C)
flow rate 0.5 mL/min
pressure 3680 psi (254 bar)
sample 500 ng/mL in 50:50, water:methanol
injection 2 µL
detector MS, ESI-, combined SIR, 699.7, 701.7 m/z

Description

Analysis Note Phosphatidylethanol and its metabolites in blood are markers of ethanol consumption. Shown here is the rapid resolution of the metabolites on a Titan PFP column.
Categories Analytical Chromatography, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Titan is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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