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LC/MS Analysis of Phosphatidylethanol Metabolites on Ascentis® Express RP-Amide

LC/MS Analysis of Phosphatidylethanol Metabolites on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.0 µm particles (51569-U)
column temp. 50 C
gradient 5 mM ammonium formate in [A] water; [B] methanol; [C] acetonitrile; (10:45:47, A:B:C)
flow rate 0.5 ml/min
pressure 2745 psi (189 bar)
sample 500 ng/mL in 50:50, water:methanol
injection 2 µl
detector MS, ESI-, combined SIR: 699.7, 701.7 m/z

Description

Analysis Note Phosphatidylethanol and its metabolites in blood are markers of ethanol consumption. Shown here is the rapid resolution of the metabolites on an Ascentis® Express RP-Amide column.
Categories Analytical Chromatography, Drugs of Abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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