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LC/MS Analysis of Pilocarpine and Betaxolol in the Presence of Benzalkonium Chlorides on Ascentis® Express HILIC

LC/MS Analysis of Pilocarpine and Betaxolol in the Presence of Benzalkonium Chlorides on Ascentis® Express HILIC

Conditions

column Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 µm particles (53981-U)
column temp. 35 C
mobile phase [A] 2 mM arrmonium acetate, pH 6.7 in [B] 10:90 (v/v) water:acetonitrile
flow rate 1.0 ml/min
pressure 1160 psi (80 bar)
sample 10 µg/mL each in acetonitrile
injection 2 µl
detector ESI(+), XlC, m/z 304, 332 (BACs), m/z 209 (pilocarpine), m/z 308 (betaxolol)

Description

Categories Analytical Chromatography, Beta-blockers, Cholinergic agonist
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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