LC/MS Analysis of Polypeptide Drugs on Ascentis® Express RP-Amide

LC/MS Analysis of Polypeptide Drugs on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 µm particles (53913-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium acetate in water, pH 4.2; [B] 10 mM ammonium acetate in 90:10 (v/v) acetonitrile:water, pH 4.7
gradient held at 20% B for 0.1 min; 20 to 80% B in 2.9 min; 80 to 20% B in 0.01 min; held at 20% B for 0.99 min
flow rate 0.3 mL/min
sample 1 µL/mL in 10mM ammonium formate, 80:20, acetonitrile:water
injection 3 µL
detector TOF, ESI(+), m/z 100-2500

Description

Analysis Note This application demonstrates the suitability of the Ascentis Express RP Amide for the analysis of polypeptide drugs.
Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links