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LC/MS Analysis of Seven Acyl-L-Carnitines on Ascentis® Express OH5 (HILIC Mode)

LC/MS Analysis of Seven Acyl-L-Carnitines on Ascentis® Express OH5 (HILIC Mode)

Conditions

column Ascentis(R) Express OH5, 15 cm x 2.1 mm, 2.7 µm particles (53764-U)
column temp. 30 C
mobile phase [A] 50 mM ammonium formate, pH 3; [B] acetonitrile
gradient 2% A for 0.2 min, to 26% A in 14.8 min; to 50% A in 0.1 min; held at 50% A for 4.9 min; to 2% A in 0.1 min
flow rate 0.4 ml/min
pressure 3626 psi (250 bar)
sample each compound, 0.5 ng on column, except carnitine, 1 ng on column
injection 0.5 µl
detector UV, 210 - 600 nm; MS, ESI(+)

Description

Analysis Note Carnitine plays an important role in fatty acid metabolism. A prerequisite for studies on the wide variety of acyl-L-carnitines and their function is the availability of acyl-L-carnitine standards and a reliable and sensitive analytical methodology. LC/MS using hydrophilic interaction liquid chromatography (HILIC) on an Ascentis Express OH5 column is shown here for the separation of seven acyl-L-carnitines without derivatization.
Categories Analytical Chromatography, Acylcarnitines
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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