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LC/MS Analysis of Vaccinium Antioxidants on Ascentis® Express C18

LC/MS Analysis of Vaccinium Antioxidants on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm X 2.1 mm I.D., 2.7 µm particles (53823-U)
column temp. 35 C
mobile phase [A] 0.1% formic acid in water; [B] 0.1% formic acid in 75:25 (v/v) acetonitrile:water; (78:22, A:B)
flow rate 0.3 ml/min
pressure 3260 psi (225 bar)
sample 20 µg/mL in 0.1% formic acid
injection 5 µl
detector ESI(+), TOF

Description

Analysis Note Fruits of the genus Vaccinium are popular sources for anthocyanidin antioxidants. This genus includes the commonly known blueberry, huckleberry, and bilberry.
Categories Analytical Chromatography, Antioxidants
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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