LC-MS Anlaysis of Androsterone Isomers on Ascentis® Express Biphenyl

LC-MS Anlaysis of Androsterone Isomers on Ascentis® Express Biphenyl

Conditions

column Ascentis Express Biphenyl, 10cm x 2.1mm, 2.7mum particles (64065-U)
column temp. 35 C
mobile phase [A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid 50:50 (A:B)
flow rate 0.2 ml/min
pressure 4200 psi (290 bar)
sample 500 ng/mL in mobile phase
injection 2 µl
detector MS, SIM, ESI+

Description

Analysis Note This application demonstrates the separation of Androsterone and trans-Androsterone on Ascentis Express Biphenyl. The shape selectivity of the biphenyl phase makes this a suitable column for analysis with baseline resolution.
Categories Analytical Chromatography, Steroids
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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