LC/MS Extracted Ion Chromatogram of MSX, Sulfone, and Sulfoxide on Ascentis® Express OH5 (HILIC Mode)

LC/MS Extracted Ion Chromatogram of MSX, Sulfone, and Sulfoxide on Ascentis® Express OH5 (HILIC Mode)

Conditions

column Ascentis Express OH5, 10 cm x 3 mm I.D., 2.7 µm particles (53769-U)
column temp. ambient
mobile phase [A] 0.1% formic acid, pH 3.5 with ammonium formate; [B] acetonitrile; (25:75, A:B)
flow rate 0.4 mL/min
pressure 1350 psi (93 bar)
sample 10 µg/mL in 90% methanol
injection 5 µL
detector ESI (+), scan m/z 150-300

Description

Analysis Note This figure shows the extracted ion currents for methionine sulfoximine-related compounds on Ascentis Express OH5. The OH5 shows excellent peak shape compared to traditional HILIC on bare silica.
Categories Analytical Chromatography, Sulfur compounds, Amino Acids
Featured Industry Chemicals and Industrial Polymers
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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