LC/MS/MS Analysis of Citrinin on Ascentis® Express Phenyl-Hexyl

LC/MS/MS Analysis of Citrinin on Ascentis® Express Phenyl-Hexyl

Conditions

column Ascentis Express Phenyl-Hexyl, 5 cm x 2.1 mm I.D., 2.7 µm particles (53334-U)
column temp. 40 °C
mobile phase [A] 5mM ammonium acetate with 0.1% formic acid in water, [B] 5 mM ammonium acetate with 0.1% formic acid in methanol
gradient 50% to 95% B in 2 min, held at 95% B for 1 min, to 50% B in 0.1 min, held at 50% B for 0.9 min
flow rate 0.4 mL/min
pressure 3263 psi (225 bar)
sample acetonitirle:water (50:50)
injection 2 µL
detector MS/MS ESI(+), MRM, 251.1 > 233.1 (Citrinin), 264.1 > 246.1 (13C-Citrinin)

Description

Analysis Note Using Ascentis Express Phenyl-Hexyl, a fast rugged analytical method was established for citrinin.
Categories Analytical Chromatography, Mycotoxins
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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