LC/MS/MS Analysis of Fentanyl and Fentanyl Analogs on Ascentis® Express Biphenyl

LC/MS/MS Analysis of Fentanyl and Fentanyl Analogs on Ascentis® Express Biphenyl

Conditions

column Ascentis Express Biphenyl, 5 cm x 2.1 mm I.D., 2.7 µm particles (64057-U)
column temp. 50 C (ambient)
mobile phase [A] 0.1% formic acid in water ; [B]0.1% formic acid in methanol
gradient 20%B to 40%B in 1 min, then to 50%B in 1 min, then to 80%B in 1 min, then hold 80%B for 1min, then to 20% in 0.1 min, then hold 20% B for 1.4 min
flow rate 0.6 ml/min
pressure 5076 psi (350 bar)
sample 250 pg/mL in 90:10 methanol:water
injection 1 µl
detector MS/MS, ESI(+), MRM (See Figure for transitions)

Description

Analysis Note Fast and selective method for fentanyl and fentanyl analogs
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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