LC/MS/MS Analysis of Immunosuppressant Drugs on apHera™ C18

LC/MS/MS Analysis of Immunosuppressant Drugs on apHera™ C18

Conditions

column apHera C18, 15 cm x 2.1 mm I.D., 5 µm particles (56100AST)
column temp. 65 °C
mobile phase [A] 0.1% formic acid water; [B] 0.1% formic acid in 98:2 (v/v) acetonitrile:water
gradient 50% B for 2 min; to 100% B in 2 min; held for 2 min
flow rate 0.4 mL/min
sample 300 ng/mL in (4:1) 1% formic acid acetonitrile:water
injection 5 µL
detector MS, ESI(+), MRM

Description

Analysis Note The apHera C18 HPLC column packed with polymeric particles demonstrated excellent peak shape and rapid elution for these bulky, immunosuppressant compounds under conditions not permitted by silica-based HPLC columns. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Categories Analytical Chromatography, Clinical, immunosuppressants, Immunosuppressants
Featured Industry Clinical Testing
Pharmaceutical (small molecule)
Legal Information apHera is a trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS, application for HPLC

Materials

     
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