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LC-MS/MS Analysis of Melamine Extracted from Milk on Ascentis® Express HILIC

LC-MS/MS Analysis of Melamine Extracted from Milk on Ascentis® Express HILIC

Conditions

column Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 µm particles (53939-U)
column temp. 30 C
mobile phase [A] 10 mM ammonium formate in 10:90 (v/v) water:acetonitrile; [B] 10 mM ammonium formate in 30:70 (v/v) water:acetonitrile
gradient held at 0% B for 5 min; 0 to 100% B in 0.1 min; held at 100% B for 4.9 min; 100 to 0% B in 0.1 min; held at 0% B for 4.9 min
flow rate 0.2 ml/min
sample SPE extract from spiked milk
injection 2 µl
detector ABI 3200 Q-Trap

Description

Categories Analytical Chromatography, Food & Beverage Analysis, Beverage Testing, Amines, Milk
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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