LC/MS/MS Analysis of Multiple Mycotoxins on Ascentis® Express Phenyl-Hexyl

LC/MS/MS Analysis of µLtiple Mycotoxins on Ascentis® Express Phenyl-Hexyl

Conditions

column Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 µm particles (53336-U)
column temp. 40 °C
mobile phase [A] 5mM ammonium formate with 0.1% formic acid in water ; [B] 5mM ammonium formate with 0.1% formic acid in acetonitrile
gradient 30 to 60% B in 3 min; to 100% B in 2 min; hold at 100% B for 2 min; 100 to 30% B in 0.5 min; hold at 30% for 2 min
flow rate 0.4 mL/min
pressure 5511 psi (380 bar)
injection 2 µL
detector MS/MS, ESI(+), MRM (See Figure for transitions)

Description

Analysis Note Fast and selective method for 16 mycotoxins.
Categories Analytical Chromatography, Mycotoxins
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for LC-MS

Materials

     
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